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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in several tumors, which leads to chromosome instability, and therefore could contribute to tumorigenesis. This protein was uncovered to affiliate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial role in promoting mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are actually described. Two supplemental splice variants have been reported but comprehensive nucleotide sequence information and facts will not be but readily available.
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Blocking peptides are peptides that bind precisely for the focus on antibody and block antibody binding. These peptide usually is made up of the epitope regarded from the antibody.
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This manual outlines further probable optimization of cellular and immunoassay parameters to be sure the best possible final results are...
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This guidebook outlines even more doable optimization of mobile and immunoassay parameters to guarantee the very best effects are...
Antibodies sure to the blocking peptide no more bind to your epitope to the focus on protein. By comparing the staining from your blocked antibody compared to the antibody on your own, one can see which staining is specific.
Concentrate on Information and facts Cyclin E belongs to your remarkably conserved cyclin relatives, whose associates are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add to your temporal coordination of every mitotic occasion. Cyclin E sorts a fancy website with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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